Figure 5. The Golgi/endosome and vacuole subcomplexes are functionally independent.

(A) Subcellular localizations of GFP-Yif1 before (0 hr) and after (4 hr) amino acid starvation. (B) Western blot analysis of the GFP-Yif1 starvation assay. 1 OD₆₀₀ cells were loaded in each lane. (C) Subcellular localizations of Cot1-GFP before (0 hr) and after (8 hr) Zn²⁺ withdrawal. (D) Western blot analysis of the Cot1-GFP degradation assay. Same volume of cells was loaded, with 0.25 OD₆₀₀ cells loaded at 0 hr. Scale bar: 2 µm.