Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background (54 yeast strains) | please find in Supplementary file 1 | |||
Transfected construct (11 yeast constructs) | please find in Supplementary file 1 | |||
Antibody | rabbit anti-G6PDH | Sigma-Aldrich | A9521 | |
Antibody | mouse anti-GFP | Santa Cruz Biotechnology, Inc. | SC9996 | |
Antibody | mouse anti-Flag | Sigma-Aldrich | F7425 | |
Antibody | rabbit anti-HA | Life technologies | 715500 | |
Antibody | mouse anti-HA | Sigma-Aldrich | 12CA5 | |
Antibody | mouse anti-Vph1 | Invitrogen | 10D7 | |
Antibody | rabbit anti-GFP | Torrey Pines Biolabs | TP401 | |
Antibody | M2 anti-FLAG resin | Sigma-Aldrich | A2426 | |
Antibody | anti-HA resin | Sigma-Aldrich | E6779 | |
Peptide | 3xFlag peptide | Sigma-Aldrich | F3290 | |
Chemical compound | Rapamycin | LC Laboratories | ASW-135 | |
Chemical compound | Doxycycline | Fisher BioReagents | 162209 | |
Chemical compound | zymolyase | Amsbio | 120493–1 | |
Chemical compound | FM4-64 | Invitrogen | T3166 | |
Chemical compound | Protease Inhibitor Cocktail | Roche | 21169500 |