Figure 6. Effect of exogenous leptin administration and high-fat feeding on food intake in ACC DKI mice.

(A) 24 hr food intake after intraperitoneal injection with saline or leptin (1 μg/g body weight) twice daily at the onset of the dark and light cycle (n = 8); *p<0.05 genotype differences, p<0.01 treatment effect as determined by 2-way repeated measures ANOVA with Bonferroni post-hoc test. (B) Hypothalamic STAT3 phosphorylation (pTyr⁷⁰⁵) 45 min after intraperitoneal injection with saline or leptin (1 μg/g body weight). Lysates from saline- and leptin-injected mice were run on separate gels, but transferred onto the same membrane for immunoblotting. STAT3 blots were cropped to remove non-specific signals from higher molecular weight proteins in the lysate. (C) Quantification of STAT3 phosphorylation normalized to STAT3 total protein signal from the same membrane (n = 3 saline-injected mice, n = 8–9 leptin-injected mice); p<0.01 represents treatment effect as determined by 2-way ANOVA with Bonferroni post-hoc test. (D) Body mass increase of wild-type and ACC DKI mice during 15 weeks of high-fat feeding (n = 9); data were analyzed by 2-way repeated measures ANOVA with Bonferroni post-hoc test. (E) Average 24 hr food intake measured over four consecutive days (n = 5); data were analyzed by unpaired t-test, two-tailed. (F) Accumulated food intake at 2 hr and 5 hr after intraperitoneal injection with saline or ghrelin (1 μg/g body weight) (n = 9) and (G) accumulated food intake at 2 hr and 5 hr after overnight (16 hr) fast (n = 9); data were analyzed by 2-way repeated measures ANOVA with Bonferroni post-hoc test; p<0.0001 showing differences in food intake at different time points after re-feeding. All data are presented as mean ± s.e.m.