Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Acaca | NA | MGI:108451; Ensembl:ENSMUSG00000020532 | |
| Gene (Mus musculus) | Acacb | NA | MGI:2140940; Ensembl:ENSMUSG00000042010 | |
| Genetic reagent (Mus Musculus, males) | ACC DKI | doi: 10.1038/nm.3372 | MGI:5780965; MGI:5780967 | C57BL/6-Acacatm1.1Grst, C57BL/6-Acacbtm1.1Grst; maintained in B.E. Kemp laboratory |
| Antibody | anti-pSTAT3 (Tyr705) (rabbit monoclonal) | Cell Signaling | 9145 | 1:1000 in PBST |
| Antibody | anti-STAT3 (mouse monoclonal) | Cell Signaling | 9139 | 1:3000 in PBST |
| Antibody | anti-pACC (ACC1 Ser79/ACC2 Ser212) (rabbit polyclonal) | Cell Signaling | 3661 | 1:1000 in PBST |
| Antibody | anti-AMPK panα (rabbit monoclonal) | Cell Signaling | 5831 | 1:2000 in PBST |
| Antibody | anti-α,β tubulin (rabbit polyclonal) | Cell Signaling | 2148 | 1:5000 in PBST |
| Antibody | anti-AMPKα (Thr172) (rabbit monoclonal) | Cell Signaling | 2535 | 1:1000 in PBST |
| Antibody | anti-UCP1 (rabbit polyclonal) | Alpha Diagnostic | UCP11-A | 1:1000 in PBST |
| Antibody | anti-ACC1 (sheep polyclonal) | doi: 10.1038/nm.3372 | 1:50 in PBS (conjugated to Protein A agarose); against CDEPSPLAKTLELNQ (rat Acc1 (1–15 Cys15); G. Hardie laboratory (University of Dundee) | |
| Antibody | anti-c-Fos (rabbit polyclonal) | Millipore | ABE457 | 1:1000 in PBS (0.2% Triton X-100, 0.1% BSA, 2% normal swine serum) |
| Antibody | anti-AMPK α1 (rabbit polyclonal) | doi: 10.1016/j.chembiol.2008.10.005. | 1 μg in PBS (conjugated to Protein A agarose); against CARHTLDELNPQKSKHQG-COOH (AMPK α1 (373–390 Cys373); B.E. Kemp laboratory | |
| Antibody | anti-AMPK α2 (rabbit polyclonal) | doi: 10.1016/j.chembiol.2008.10.005. | 1 μg in PBS (conjugated to Protein A agarose); against CMDDSAMHIPPALKPH-NH2) (AMPK α2 (351–366 Cys351); B.E. Kemp laboratory | |
| Antibody | anti-rabbit immunoglobulin (swine polyclonal, biotinylated) | Dako | E0353 | 1: 500 in PBS (0.2% Triton X-100,0.1% BSA, 2% normal swine serum) |
| Antibody | anti-rabbit immunoglobulin (goat polyclonal, HRP-conjugated) | Dako | P0448 | 1:3000 in PBST |
| Peptide, recombinant protein | Murine leptin | Lonza (Australia); Peprotech (Rocky Hill, NJ) | 450–31 | 1 μg/g body weight in saline |
| Peptide, recombinant protein | n-octanoylated murine ghrelin | Purar Chemicals | 1 μg/g body weight in saline (acute);30 μg/mouse at 0.5 μl/h in saline (osmotic pumps) | |
| Commercial assay or kit | Rat/mouse ghrelin ELISA kit | Millipore | EZRGRA-90K | |
| Commercial assay or kit | Mouse leptin ELISA kit | Millipore | EZML-82K | |
| Commercial assay or kit | Mouse insulin ELISA kit | Mercodia | 10-1247-01 | |
| Commercial assay or kit | Thyroxine (T4) ELISA Kit | Invitrogen | EIAT4C | |
| Commercial assay or kit | Epinephrine/norepinephrine ELISA kit | Abnova | KA3767 | |
| Commercial assay or kit | NEFA C kit | Wako | 279–75401 | |
| Commercial assay or kit | LabAssay Triglyceride kit | Wako | 290–63701 | |
| Chemical compound, drug | [D-Lys3]-GHRP-6 | Abcam | ab141148 | 6.7 μmol/kg in saline |