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. 2018 Feb 13;7:e32656. doi: 10.7554/eLife.32656

Key resources table.

Reagent type (species)
or resource
Designation Source or reference Identifiers Additional information
Gene (Mus musculus) Acaca NA MGI:108451; Ensembl:ENSMUSG00000020532
Gene (Mus musculus) Acacb NA MGI:2140940; Ensembl:ENSMUSG00000042010
Genetic reagent (Mus Musculus, males) ACC DKI doi: 10.1038/nm.3372 MGI:5780965; MGI:5780967 C57BL/6-Acacatm1.1Grst, C57BL/6-Acacbtm1.1Grst; maintained in B.E. Kemp laboratory
Antibody anti-pSTAT3 (Tyr705) (rabbit monoclonal) Cell Signaling 9145 1:1000 in PBST
Antibody anti-STAT3 (mouse monoclonal) Cell Signaling 9139 1:3000 in PBST
Antibody anti-pACC (ACC1 Ser79/ACC2 Ser212) (rabbit polyclonal) Cell Signaling 3661 1:1000 in PBST
Antibody anti-AMPK panα (rabbit monoclonal) Cell Signaling 5831 1:2000 in PBST
Antibody anti-α,β tubulin (rabbit polyclonal) Cell Signaling 2148 1:5000 in PBST
Antibody anti-AMPKα (Thr172) (rabbit monoclonal) Cell Signaling 2535 1:1000 in PBST
Antibody anti-UCP1 (rabbit polyclonal) Alpha Diagnostic UCP11-A 1:1000 in PBST
Antibody anti-ACC1 (sheep polyclonal) doi: 10.1038/nm.3372 1:50 in PBS (conjugated to Protein A agarose); against CDEPSPLAKTLELNQ (rat Acc1 (1–15 Cys15); G. Hardie laboratory (University of Dundee)
Antibody anti-c-Fos (rabbit polyclonal) Millipore ABE457 1:1000 in PBS (0.2% Triton X-100, 0.1% BSA, 2% normal swine serum)
Antibody anti-AMPK α1 (rabbit polyclonal) doi: 10.1016/j.chembiol.2008.10.005. 1 μg in PBS (conjugated to Protein A agarose); against CARHTLDELNPQKSKHQG-COOH (AMPK α1 (373–390 Cys373); B.E. Kemp laboratory
Antibody anti-AMPK α2 (rabbit polyclonal) doi: 10.1016/j.chembiol.2008.10.005. 1 μg in PBS (conjugated to Protein A agarose); against CMDDSAMHIPPALKPH-NH2) (AMPK α2 (351–366 Cys351); B.E. Kemp laboratory
Antibody anti-rabbit immunoglobulin (swine polyclonal, biotinylated) Dako E0353 1: 500 in PBS (0.2% Triton X-100,0.1% BSA, 2% normal swine serum)
Antibody anti-rabbit immunoglobulin (goat polyclonal, HRP-conjugated) Dako P0448 1:3000 in PBST
Peptide, recombinant protein Murine leptin Lonza (Australia); Peprotech (Rocky Hill, NJ) 450–31 1 μg/g body weight in saline
Peptide, recombinant protein n-octanoylated murine ghrelin Purar Chemicals 1 μg/g body weight in saline (acute);30 μg/mouse at 0.5 μl/h in saline (osmotic pumps)
Commercial assay or kit Rat/mouse ghrelin ELISA kit Millipore EZRGRA-90K
Commercial assay or kit Mouse leptin ELISA kit Millipore EZML-82K
Commercial assay or kit Mouse insulin ELISA kit Mercodia 10-1247-01
Commercial assay or kit Thyroxine (T4) ELISA Kit Invitrogen EIAT4C
Commercial assay or kit Epinephrine/norepinephrine ELISA kit Abnova KA3767
Commercial assay or kit NEFA C kit Wako 279–75401
Commercial assay or kit LabAssay Triglyceride kit Wako 290–63701
Chemical compound, drug [D-Lys3]-GHRP-6 Abcam ab141148 6.7 μmol/kg in saline