Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus muscu/us) | Kif2a | PMID:1447303 | MGl:108390 | |
| Strain, strain background (Mus muscu/us) | C57BU6NJcl, C57BU6JJcl | Clea Japan | ||
| Strain, strain background(Mus muscu/us) | CreERt(CAG-cre/Esr1) | The Jackson Laboratory | Tg(CAG-cre/Esr1 *)5Amc/J (Stock No: 004453) |
|
| Strain, strain background(Mus muscu/us) | R26R (lacZ expression with the ROSA26 Cre reporter strain) | PMID:9916792 | ||
| Strain, strain background(Mus muscu/us) | Thy1-EGFP line M | The Jackson Laboratory | Tg(Thy1-EGFP)MJrs/J (Stock No: 007788) |
|
| Cell line (Mus musculus) | CMTl-1 | Merck Millipore | 129/Sv-derived ES cell (ESC) line | |
| Antibody | anti-KIF2A (mouse monoclonal) | PMID: 7535303 | (1:200) | |
| Antibody | anti-KIF2A (rabbit polyclonal) | Thermo Fisher | Thermo Fisher: PA3-16833 | (1:1500) |
| Antibody | anti-GFAP(mouse monoclonal) | BO Transduction Laboratory | BO Bioscience: 610565 (Clone 52/GFAP) |
(1:200) |
| Antibody | anti-BrdU (mouse monoclonal) for ldU | BO Pharmingen | BO Bioscience: 555627 (Clone 304) |
(1:1000) |
| Antibody | anti-BrdU (rat monoclonal) for CldU | Accurate Chemicals | Accurate Chemicals (Clone: BU1/75) |
(1:500) |
| Antibody | anti-Tau1 (mouse monoclonal) | Merck Millipore | Merck Millipore: MAB3410 (Clone PC1C6) |
(1:1000) |
| Antibody | anti-Neurofilament M (mouse monoclonal) | Sigma | Sigma: N5264 (Clone NN18) |
(1:600) |
| Antibody | anti-MAP2 (chicken monoclonal) | Abeam | Abeam: AB5392 (Clone 304) |
(1:200) |
| Antibody | anti-Synaptoporin (rabbit polyclonal) | Synapitic Systems | SYSY:102002 | (1:300) |
| Antibody | anti-Prox1 (rabbit polyclonal) | Merck Millipore | Merck Millipore: AB5475 | (1:10000) |
| Antibody | anti-Ankyrin G (mouse monoclonal) | Thermo Fisher | Thermo Fisher: 338800 (Clone 4G318) |
(1:600) |
| Antibody | Alexa 488-, 546-, 555-, 647, (Goat polyclonal) | Molecular Probes | (1: 200–1: 500) | |
| Recombinant DNA reagent | pEYFP-C1 (vector) | Clontech | ||
| Recombinant DNA reagent | YFP-Kif2a (full length) | PMID: 14980225 | Progenitors: pEYFP-C1 vector | |
| Commercial assay or kit | LA PCR Kit | Takara | TaKaRa:RR013A | |
| Chemical compound, drug | Tamoxifen | Sigma | Sigma:T5648 | (30 mg/kg body weight/day) |
| Chemical compound, drug | Pilocarpine | Sigma | Sigma: P6503 | (290 mg/kg body weight/day}, |
| Chemical compound, drug | Scopolamine methyl bromide | Sigma | sigma: 8502 | (1 mg/kg body weight/day) |
| Chemical compound, drug | ScaleView | Olympus | ||
| Chemical compound, drug | 5-iodo-2'-deoxyuridine | Sigma | Sigma: 17125 | 50 mg/kg |
| Chemical compound, drug | 5-chloro-2 '-deoxyuridene | Sigma | Sigma: C6891 | 50 mg/kg |
| Chemical compound, drug | poly-L-lysine | Sigma | Sigma: P-2636 | 100 mg/ml |
| Chemical compound, drug | Laminin | lnvitrogen | lnvitrogen: 23017–015 | 25 µg/ml |
| Software, algorithm | MicroMax 1. 3 | AccuScan Instrument | ||
| Software, algorithm | Clampex 9.2 software | Axon Instruments | ||
| Software, algorithm | IMARIS 7.2 | Bitplane:Zeiss | ||
| Software, algorithm | lmageJ 1.51 hr | NIH | ||
| Software, algorithm | SPSS V22 | IBM | ||
| Machine | MicroMax Monitor | AccuScan Instruments | ||
| Machine | Axopatch 1D amplifier | Axon Instruments |