Table 2. Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | SLC7A8 antibody | Custom made | NA | Anti-Rabbit peptide sequence: PIFKPTPVKDPDSEEQP WB: 1:1000, IHC: 1/5000 and IF:1/200 |
| s100 | Sigma-Aldrich | Ref: S2532 | IF: 1/1000 | |
| Kir4.1 | Merck Millipore | Ref: AB5818 | IF: 1/200 | |
| BA1 | Abcam | Ref: ab5076 | IF: 1/200 | |
| Phalloidin | Thermo Fisher Scientific | Ref: A22287 | IF: 1/100 | |
| Donkey anti-Goat Alexa Fluor 546 |
Thermo Fisher Scientific | Ref: A-11056 | IF: 1/300 | |
| Donkey anti-Rabbit Alexa Fluor 488 |
Thermo Fisher Scientific | Ref: A-21206 | IF: 1/300 | |
| Goat anti-Mouse Alexa Fluor 546 |
Thermo Fisher Scientific | Ref: A-11030 | IF: 1/300 | |
| Goat anti-Rabbit Alexa Fluor 488 |
Thermo Fisher Scientific | Ref: A-11034 | IF: 1/300 | |
| WGA | Thermo Fisher Scientific | Ref: W21405 | labeled with Texas-Red IF: 1 mg/mL | |
| Anti-Strep Tag GT517 | Abcam | Ref: ab184224 | IF: 1/100 | |
| Goat-anti-mouse-FITC | Abcam | Ref: ab6785 | IF: 1/300 | |
| Behavior | Rotarod | Panlab | Ref:LE8500 | |
| Treadmill | Panlab | E8710MTS | ||
| Morris water maze | Panlab | SMART camera | circular tank (150 cm diameter, 100 cm high) | |
| PPI | Panlab | LE116 | ||
| Restrain stressor | Lab Research | Ref:G05 | ||
| ABR | Tucker Davis Technologies TDT |
System 3 Evoked | ||
| Mouse | C57BL6/J wild type | Harlam | Ref: 057 | C57BL/6JOlaHsd |
| C57BL6/J wild type | Jackson laboratory | Ref: 000664/Black | ||
| Slc7a8-/- chimera | Genoway | Customized Model Development |
Strategy Figure 1—figure supplement 1 | |
| Cell Line | HeLa | Sigma Aldrich | Ref: 93021013 | |
| Chemical compound, drug |
DTT dithiothreitol | SigmaAldrich | Ref:D9779 | |
| L- [3H]-labeled alanine | Perkin Elmer | Ref: NET348250UC | 1 μCi/ml | |
| [3 hr]-tyrosine | Perkin Elmer | Ref: NET127250UC | 1 μCi/ml | |
| Commercial assay or kit | Pierce BCA Protein Assay Kit |
Thermo Scientific | Ref:23225 | |
| ECL | GE Healthcare | Ref:RPN2232 | ||
| Corticosterone EIA kit | Enzo | Ref:ADI900097 | ||
| A + B conjugate | Vectastain | Ref: ABC kit | ||
| Rneasy | Qiagen | Ref: 74104 | ||
| High-capacity cDNA Reverse Transcription Kit |
Applied Biosystems | Ref: 4368813 | ||
| TaqMan Gene Expression Assay |
Applied Biosystems | potassium voltage-gated channel subfamily Q member 2 (Kcnq2) Mm00440080_m1; potassium voltage-gated channel subfamily Q member 3 (Kcnq3) Mm00548884_m1; potassium voltage-gated channel subfamily Q member 5 (Kcnq5) Mm01226041_m1; prestin (Slc26a5) Mm00446145_m1; T-box transcription factor TBX18 (Tbx18) Mm00470177_m1; interleukin one beta (Il1b) Mm00434228m1; interleukin 6 (Il6) Mm00446190m1; solute carrier family 7 (cationic amino acid transporter, y + system), member 8 (Slc7a8) Mm01318971m1 | ||
| QuikChange site-directed mutagenesis kit |
Stratagene | Ref: 200524 | ||
| Gene (human) | SLC7A8 | NCBI | NM_012244.3 | Protein NP_036376.2 (535AA) |
| Slc7a8 | NCBI | NM_016972.2 | Protein NP_058668.1 (531AA) | |
| Sequence-based reagent |
whole genome sequence |
Illumina | HiSeq 2000 | Data coverage was ranging from 4 to 10X |
| Sanger sequencing | Life Technologies | 3500 Dx Genetic Analyzer | ||
| BigDye | Life Technologies | ABI PRISM 3.1 Big Dye terminator |
||
| Software, algorithm | BioSig | Tucker Davis Technologies TDT |
NA | |
| Graph Pad Software | GraphPad Software, Inc |
Prism 4 | https://www.graphpad.com/scientific-software/prism/ | |
| SeqMan Pro software | DNAstar | https://www.dnastar.com/t-seqmanpro.aspx | sequencing assembly and analysis | |
| Annotations tools | ANNOVAR | http://annovar.openbioinformatics.org/en/latest/ | functional annotation of genetic variants DOI:10.1093 | |
| Genome Research | Bcftools | http://samtools.github.io/bcftools/ | ||
| SPSS 23.0 statistic software package |
IBM | NA | https://www.ibm.com/analytics/data-science/predictive-analytics/spss-statistical-software | |
| Transfected construct | Slc7a8 construct | Agilent | Catalog #212205 | Resistances: Neomycin and thymidine kinase |
| pcDNA3.1-StrepTag | ThermoFisher | Ref: V79020 | fused SLC7A8 or SLC3A2 | |