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. Author manuscript; available in PMC: 2018 Feb 13.
Published in final edited form as: Neuron. 2015 Mar 4;85(5):1086–1102. doi: 10.1016/j.neuron.2015.02.006

Figure 5. Nms−/−/Nmu−/− Mice Exhibit Normal Freerunning Circadian Rhythms.

Figure 5

(A) Immunostaining of NMS (red) on SCN sections of C57BL/6J wild-type (WT) mice and Nms−/− mice. NMS immunoreactivity was not detected in Nms−/− mice. The scale bar is 50 μm.

(B) Representative actograms of WT (top-left panel), Nms−/− (top-right), Nmu−/− (bottom-left), and Nms−/−/Nmu−/− mice (bottom-right).

(C) Quantification of circadian activity in WT, Nms−/−, Nmu−/−, and Nms−/−/Nmu−/− mice. Mean freerunning periods and amplitudes were not significantly different between genotypes (one-way ANOVA, p>0.05 by Tukey’s post-hoc test). Total activity counts of Nmu−/− mice were significantly greater than that of Nms−/−/Nmu−/− mice (one-way ANOVA, *p<0.05 by Tukey’s post-hoc test). Values are mean ± SEM (WT, n=5; Nms−/−, n=8; Nmu−/−, n=5; Nms−/−Nmu−/−, n=13).