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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: DNA Repair (Amst). 2018 Jan 9;62:18–27. doi: 10.1016/j.dnarep.2018.01.004

Figure 2. An AP site blocks Taq-mediated primer extension.

Figure 2

Taq polymerase extension reactions were performed as described in the text on 40-mer oligonucleotides containing either an 8-oxo dG residue (8oxo) or an AP site (AP), see methods section for details, in the absence (-) or presence (+) of complementary primer Z (15-mer, see methods). The products were resolved by electrophoresis, stained with ethidium bromide and subject to scanning densitometry. The relative amount of full-length product (40 nt) was quantitated using image J and plotted on the y axis (arbitrary units).