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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: DNA Repair (Amst). 2018 Jan 9;62:18–27. doi: 10.1016/j.dnarep.2018.01.004

Figure 3. 8-oxo-dG repair efficiency is similar in wild-type and NER-deficient Chinese hamster clones.

Figure 3

An oligonucleotide containing an 8-oxo-dG modification (O, in red) is annealed to single-stranded M13 and a primer extension reaction performed as described in the Methods. Covalently closed circular double-stranded DNA is gel-purified, and transfected into wild-type V79 (blue) and NER-deficient V-H1 (red) Chinese hamster cells via lipofection. Low molecular weight DNA was recovered 1, 1.5 and 3-hours post-transfection and treated with OGG1 to convert unrepaired 8-oxo-dG residues to abasic sites. DNA repair assays were then performed as described in the legend to Figure 1. Values depict mean percent repair, ± SEM, N=3.