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. Author manuscript; available in PMC: 2019 Feb 15.
Published in final edited form as: Virus Res. 2017 Dec 30;246:1–11. doi: 10.1016/j.virusres.2017.12.013

Figure 5. Ubiquitinated NS1 has reduced affinity for NS4B.

Figure 5

A) Schematic representation of the experimental approach. B–D) HEK293T cells were transfected with HA-NS4B or NS1 with or without the deubiquitinase Flag-OTU or the catalytically inactive mutant Flag-OTU2A. Cells were lysed and whole cell extracts (WCE) were used for immunoblot (B). Then, the NS4B expressing WCE were mixed with WCE containing ubiquitinated or deubiquitinated NS1 (C) and immunoprecipitation (IP) of NS4B was performed using anti-HA beads (D). The indicated proteins were detected by immunoblots (IB). The experiment shown is a representative of 2 independent experiments.