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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Transl Res. 2017 Oct 28;192:1–14. doi: 10.1016/j.trsl.2017.10.005

Figure 3. The axon-growth supportive milieu of Aldh1a1−/− secretome is RA-dependent.

Figure 3

DRG neurons were cultured in DRG culture medium with the secretome from Aldh1a1−/− adipocytes (1/1, v/v) that were treated with or without RA (100nM). RA was added to Aldh1a1−/− differentiation medium (Day 0, 2, 5). To prepare this secretome, medium was collected for the last 24 hours. (A) Representative images were selected from 3 independent experiments. (B) Mean neurite growth indices were measured by XTI microscopy in DRG neurons in DRG culture medium containing Veh (vehicle, white bar), NGF (vertical lined bar), or Aldh1a1−/− secretome treated without (black bar) or with RA (hatched bar). An asterisk indicates P<0.05; Mann-Whitney U test.