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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Transl Res. 2017 Oct 28;192:1–14. doi: 10.1016/j.trsl.2017.10.005

Figure 5. Secretome of grafted encapsulated Aldh1a1−/−adipocytes promotes innervation in WAT in WT obese mice.

Figure 5

Obese WT female mice (n=18) on a HF diet were treated with acellular (n=3) microcapsules or micro capsules containing WT (n=5) or Aldh1a1−/− adipocytes (n=5) or mice remained untreated (n=5) (Study 3). (A) Immunohistochemical analysis of peripherin protein levels in axons in iAb WAT of the animal groups in Study 3. (A) Representative images of peripherin (brown) immunoreactive areas of nerves in paraffin-embedded iAb fat from mice injected with vehicle and encapsulated WT and Aldh1a1−/− cells at 20× magnification. Green arrows indicate examples of peripherin-positive axons of nerves at 40× magnification. ‘C’ indicates empty core of microcapsules because encapsulated cells are attached at the inner surface of capsules. The ‘A’ letters show examples of adipocytes. (B) Peripherin was analyzed by Western blot in whole homogenized iAb WAT pads from mice in Study 3. Bars show peripherin levels from the same tissues normalized to β-actin levels (n=4). An asterisk indicates a significant difference between empty and Aldh1a1−/− groups (p<0.05; Mann-Whitney U test). (C) Immunohistochemical analysis of tyrosine hydroxylase (TH, brown) protein levels in axons of the animal groups in Study 3. Note that TH-positive areas are found within nerves (40×) (red arrow) or in areas containing numerous multilocular adipocytes.