Table 1.
Systemic and renal characteristics in wild type (WT) and GOF pigs fed a normal (ND) or atherogenic (AD) diet for 6-month.
| Parameter | WT-ND | WT-AD | PCSK9 GOF-ND | PCSK9 GOF-AD | |||
|---|---|---|---|---|---|---|---|
|
| |||||||
| 3-months | 6-months | 6-months | 3-months | 6-months | 3-months | 6-months | |
| Body Weight (Kg) | 50.7±3.1 | 65.6±4.6# | 87.0±8.3 | 33.8±4.0 | 46.7±3.3# | 40.9±1.3 | 58±2.4# |
| MAP (mmHg) | 118.2±5.0 | 119.8±9.8 | 118.6±10.6 | 132.8±4.0* | 137.3±8.0* | 131.9±2.8* | 133.8±4.4* |
| SVR (dynes·s/cm5) | 2812±617 | 2900±514 | 2441±645 | 3794±433* | 3592±520*¥ | 3570±418* | 3680±551*¥ |
| Lipid fractions (mg/dL) | |||||||
| Total Cholesterol | 76.7±24.5 | 83.71±20.9 | 312.3±13.0* | 466.7±214.2* | 434.4±47.2* | 857.6±143.0*† | 952.1±175.6*† |
| HDL-C | 39.57±6.45 | 47.0±13.4 | 136.7±15.2*† | 45.1±19.9 | 37.6±3.9 | 80.3±25.3*† | 87.1±20.8*† |
| LDL-C | 32.1±15.4 | 29.8±12.6 | 160.2±5.3*† | 412.0±195.2* | 388.8±45.6* | 769.5±121.5*† | 853.5±161.1*† |
| VLDL-C | 2.9±1.3 | 3.92±2.0 | 15.5±4.4*† | 7.1 ±1.9* | 6.4±2.1 | 6.2 ±1.8* | 9.21±1.6*† |
| Triglycerides | 18.3±8.4 | 24.5±12.7 | 77.3±22.0* | 44.2±11.6* | 40.3±13.5 | 38.9±11.5* | 57.6±10.1*† |
| Albumin/Creatinine Ratio | - | 27.33±14 | - | - | 35.2±25 | - | 29.6±18 |
| PCSK9 (ng/mL) | - | 0.01±0.03 | 0.01±0.02 | - | 724±54*¥ | - | 743±70*¥ |
| Perfusion (mL/min/cc) | |||||||
| Cortex | 4.2±0.3 | 3.7±0.2 | 5.8±0.9 | 4.2±0.3 | 4.4±0.2 | 4.2±0.4 | 4.1±0.6 |
| Medulla | 2.8±0.3 | 1.8±0.2 | 3.6±0.7 | 2.6±0.2 | 3.0±0.4 | 2.8±0.6 | 3.2±0.7 |
| RBF (ml/min/cc) | 4.1±0.23 | 4.2±0.4 | 5.6±0.5 | 4.0±0.2 | 4.1±0.1 | 4.1±0.3 | 4.0±0.4 |
| GFR (ml/min/kg) | 0.66±0.02 | 0.63±0.03 | 0.57±0.03 | 0.70±0.05 | 0.67±0.03 | 0.60±0.02 | 0.63±0.02 |
| Tubular Injury Score | - | 1.2±0.58 | - | - | 1.86±0.69* | - | 1.57±0.53* |
ANOVA yielded p<0.05 for all systemic measurements, but not for renal parameters.
*
p<0.05 vs. WT-ND,
¥
p<0.05 vs. WT-AD
†
p<0.05 vs. PCSK9 GOF-ND in the same time period,
#
p<0.05 vs. Baseline (n=7 each group, except WT-ND with n=3).
GOF: Gain of function. MAP: Mean arterial pressure, RBF: Renal blood flow, GFR: glomerular filtration rate. SVR: Systemic vascular resistance, NS: Not significant. VLDL-C: Very low-density lipoprotein cholesterol; HDL-C: high-density lipoprotein cholesterol; LDL-C: low-density lipoprotein cholesterol.