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. 2018 Feb 13;8:2883. doi: 10.1038/s41598-018-21035-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Co-localization of glutamate and GFAP. The confocal microscopy of the brain slices were performed in a 63 × zoomed region in the striatum of the control (a) and MPTP treated mouse (b). The figure shows the cell bodies with nuclear stain DAPI (blue) having glutamate (green) and GFAP (red) immunostaining. Neuronal nuclei are bigger and are found to be co-localized with neuronal glutamate (yellow arrows), whereas, astrocytic glutamate appeared to co-localize with GFAP staining. Areas marked with white arrows show the co-localization of glutamate and GFAP in the reactive glia.