Table 2.

HPLC conditions for analysis of digested and undigested synthetic RNA oligonucleotides

Column: Waters XSelect HSS T3, 100 Å, 3.5 µm, 3.0 × 150 mm
Column temperature: 30 °C
Flow rate: 0.7 mL min−1
Injection volume: 8 μL
Step time / min	Mobile phase composition / %
	A (water)	B (acetonitrile)	C (0.2 M NH4OAc, pH 6, 25% acetonitrile)
0	89	1	10
2.5	89	1	10
5.5	80	10	10
10	40	50	10
10.1	10	80	10
11	10	80	10
11.1	89	1	10
20	89	1	10