Table 3.

HPLC conditions for analysis of digested ribosomal RNA

Column: Waters XSelect HSS T3, 100 Å, 3.5 µm, 3.0 × 150 mm
Column temperature: 30 °C
Flow rate: 0.7 mL min−1
Injection volume: 8 μL
Step time / min	Mobile phase composition / %
	A (water)	B (acetonitrile)	C(0.2 M NH4OAc, pH 6, 2.5% acetonitrile)
0	90	0	10
3.6	90	0	10
6.0	88	2	10
7.5	85	5	10
13.5	70	20	10
24	55	35	10
27.5	10	80	10
28.8	10	80	10
29.0	90	0	10
38.0	90	0	10