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. 2018 Feb 9;9:169. doi: 10.3389/fimmu.2018.00169

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Copyright © 2018 Trim, Turner and Thompson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Agreement between RT-PCR and flow cytometric assessment of macrophage abundance in lean, overweight, and obese individuals subcutaneous abdominal adipose tissue. Relative gene expression of CD68 was used to identify macrophages (n = 30) and presented as mean 2^−ΔΔCt ± SEM. Proportions of macrophages in the adipose tissue SVF as a percentage of total cells (n = 17). Macrophages were identified as CD45+HLA-DR+CD16+ cells. Presented with permission from Travers et al. (35). Abbreviations: CD, cluster of differentiation; HLA-DR, human leukocyte antigen-antigen D related; RT-PCR, reverse transcription polymerase chain reaction; SVF, stromal vascular fraction.