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. 2018 Feb 9;9:111. doi: 10.3389/fpls.2018.00111

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Copyright © 2018 Ning, Sun, Wang, Zhang, Sun, Hu, Liu and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Modulating OsGRX20 expression influenced levels of superoxide radicals, AsA/DHA and gene transcripts associated with ROS detoxification after MeV treatment. Five-leaf plants from wild-type Xiushui11 (XS11) and Yongjing 50A (YJ50), OsGRX20-overexpression lines OE7 and OE8, and RNAi lines SE3 and SE5 were incubated in 10 μM MeV for 24 h, and the aerial tissue was subjected to analysis. (A) Relative superoxide radical level was calculated by comparison to the non-treated seedlings of individual genotypes. (B) The AsA/DHA ratio under control condition and after 24-h treatment. (C) Relative mRNA levels of four genes associated with ROS detoxification in OsGRX20-overexpression line OE7 and wild-type Xiushui 11. Data represent mean ± SD from three independent biological replicates, and an asterisk indicates a significant difference between wild-type vs. transgenic plants (^∗p < 0.05; ^∗∗p < 0.01).