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. 2018 Feb 13;8:2956. doi: 10.1038/s41598-018-20965-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ELK-generated particle size by native gel electrophoresis and TEM. (a) Lipid particles generated by incubation of peptides with DMPC lipid vesicles (lanes 1 = neu, 2 = hyd, 3 = pos, 4 = neg) and a mixture of natural lipids found in cellular membranes (lanes 5 = neu, 6 = hyd, 7 = pos, 8 = neg). Both types of lipids were suspended in PBS at 1 mg/ml along with 0.5% PE-rhodamine. The vesicles were incubated with 0.5 mg/ml peptides in PBS at room temperature for 2 hours with gentle shaking. The lipid particles formed by peptides were separated in a 1D native TBE gel and scanned using a Typhoon scanner. (b) Particle size distribution after reconstitution of neu with phospholipid and cholesterol (neu:POPC = 1:6.25 and cholesterol:POPC = 1:10). Mean size of particles is 9.2 nm. Inset shows a TEM image of a representative particle made with neu. Scale 10 nm, magnification 50000X. Uncropped images of Fig. 3 are provided in Supplementary Figures 2, 3 and 4.