Fig. 2.

Localization of post-translationally labeled Nb_G-tagged VSRs. a Cycling Nb_G-tagged red fluorescent VSRs are b post-translationally labeled by endocytosed GFP. c GFP-labeled red fluorescent Nb_G-tagged VSRs. d Pearson’s (r_P) and Spearman’s (r_S) correlation (PSC) coefficients of Nb_G-RFP-VSRs and labeling GFP. Data are presented as average ± s.e.m. of 10 individual cells. The graph shows a representative sample of two independent experiments. e, f Colocalization of post-translationally GFP-labeled non-fluorescent cycling Nb_G-tagged VSRs (Nb_G-VSR) with red fluorescent compartmental markers (CM) for g, i the TGN/EE, h, i MVBs/LEs and vacuole, j, l the trans−/ and k, l cis-Golgi and m the ER. i, l PSC coefficients of the labeled Nb_G-VSR and coexpressed markers RFP-SYP61, Aleu-RFP, Man1-RFP and ST-RFP are calculated and presented as in d. Graphs show a representative sample of two independent experiments. Labeled Nb_G-VSRs colocalize with the TGN/EE marker but not with markers for MVB/LE and vacuole and the cis-/trans-Golgi. i Significance was calculated using unpaired, two tailed t-test (n = 10, ***P < 0.001). Scale bars 10 µm, insets 5 µm. Insets show magnifications