Figure 5.

The Homer1a/AMPK/autophagy pathway reduces H₂O₂-induced oxidative stress. HT-22 cells were transfected with LV-NC or LV-Homer1a for 48 h. After transfection, the cells were treated with compound C (20 μM) for 24 h then H₂O₂ applied for 24 h. The expression of p-AMPK, AMPK, Beclin1 and LC3II were detected by Western blot analysis (A–D). Cytotoxicity was detected by LDH release assay (E). ROS production and MMP were assayed (F,G). The data were expressed as means ± SEM from five experiments. ^*P < 0.05 vs. Control, ^#P < 0.05 vs. H₂O₂+LV-NC, ^$P < 0.05 vs. H₂O₂+LV-Homer1a.