Figure 4.

Number of β III-tubulin-positive cells. (A) ReNcell ventral mesencephalon cells treated with bisphenol A (BPA), β-estradiol (E2), or control were immune-stained with antibodies for β III-tubulin (red) and glial fibrillary acidic protein (GFAP) (green). Nuclei were identified by Hoechst 33342 staining. Panel (B) shows the ratio of β III-tubulin-positive cells per total cells. The number of β III-tubulin-positive cells was counted with four arbitrarily selected fields per well (scale bar: 200 µm). Data are represented as mean ± SEM, n = 47.