Figure 5.

Negr1-KO mice show alterations in neurotransmitter receptor ligand binding in distinct hippocampal subfields. (A–C) Relative binding densities of [3H]MK-801 to NMDAR (A), [3H] Pirenzepine to M1R (B), and [3H] Oxotremorine to M2R (C). Please note that ligand binding densities for M2 AChR in the DG were in the same range of unspecific binding and have been omitted (C). Values are expressed as mean value in % ± standard error of mean (SEM), n = 8 for each genotype. Parametric data were analyzed by Student’s t-test, non-parametric data by Mann Whitney U test. (D) Western Blot showing M2R protein expression in total hippocampus lysates. GAPDH was used as a reference protein. (E) Histograms showing slightly reduced M2R/GAPDH protein band intensity ratio in hippocampus but not in cortex samples from Negr1-KO mice compared to WT (n = 6 per genotype). *p < 0.05, ***p < 0.001, Student’s t-test. Abbreviations: CA, cornu ammonis, DG, dentate gyrus, Or, stratum oriens, Rad, stratum radiatum, Py, stratum pyramidale, Mol, stratum moleculare, Gr, stratum granulare.