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. 2018 Feb 13;8:2904. doi: 10.1038/s41598-018-21120-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antigen-specific nasal immune response was decreased in Ttll1-KO mice nasally immunized with PspA-C-CPE. Ttll1-hetero (He) and -knockout (KO) mice were nasally immunized with PspA-C-CPE once a week for three weeks. One week after the final immunization, PspA-specific nasal IgA was measured by means of an enzyme-linked immunosorbent assay. Ttll1-He mice, n = 4; Ttll1-KO mice, n = 3. Data are presented as mean ± SEM and are representative of two independent experiments. Values were compared by using Welch’s t-test. *P < 0.05. OD, optical density.