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. 2018 Feb 13;8:2885. doi: 10.1038/s41598-018-21287-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Measure of whole-body glucose homeostasis, skeletal muscle insulin sensitivity and glycogen content. (a) Area under the curve (AUC) for glycaemia. (b) P-Akt and Akt expression levels in soleus skeletal muscle, measured by immunoblotting after ex vivo insulin stimulation. A representative blot is shown. Quantification values are presented as P-Akt/Akt fold induction and are expressed relative to the HF value, which was set at 1. (c) and (d) Glycogen content in skeletal muscle (c) and liver (d). (e) Hepatic triglyceride content. Data are expressed as mean ± SEM with n = 8–10 rats per group. Bars not sharing a common letter are significantly different at p < 0.05.