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. 2018 Feb 13;9:645. doi: 10.1038/s41467-018-03088-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — CAL1 positively regulates Cd contents in rice leaf and xylem sap. Leaves and xylem sap were collected from 2 weeks old seedlings exposed to 10 µM Cd for 7days, and grains was sampled from rice plants grown in Cd-contaminated paddy soil. a–c Cd levels in leaves (a), xylem sap (b) and grains (c) from NIL(TN1) and NIL(CJ06). d, e determination of Cd contents in xylem sap (d) of the CAL1-complementation lines N47-14, N47-15, and N47-18, which simulate overexpression lines due to increased expression of CAL1 (Supplementary Fig. 3d) in CJ06 background. CJ06-CK represent transgene-negative controls. e Identification of cal1 mutants generated by CRISPR/Cas9. cal1-G and cal1-T1 represent mutants with single G or T insertion in CAL1 coding region, respectively, with TN1 background. cal1-T2 and cal1-A represent mutants with single T or A insertion in CAL1 coding region, respectively, with CJ06 background. f–h Cd contents in leaves (f, g) and xylem sap (h) of cal1 mutants. TN1-CK and CJ06-CK1 represent transgene-negative controls. Data are mean ± SD, n = 3 in (a–c), or 4 in (d and f–h). Significant differences were determined by Student’s t-test (*P < 0.05, **P < 0.01)