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. 2018 Feb 13;9:645. doi: 10.1038/s41467-018-03088-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Histochemical and subcellular localization assay. a, b GUS expression driven by the CAL1^TN1 promoter. (a) Cross section of 1 week old seedling roots. b Cross section of flag leaf sheath. c, d Fluorescence images of cross-sectioned seedling roots (c) and flag leaf sheath (d) from plants expressing the construct proCAL1^TN1::CAL1-mRFP. e–g Subcellular localization of CAL1. Onion epidermal cells transiently transformed with mRFP (e), CAL1-mRFP fusion (f), or mRFP-CAL1 (g) were incubated in 40% sucrose to induce plasmolysis and then imaged by confocal microscopy. h Subcellular localization of CAL1 in rice plants harboring the construct 35S::CAL1-mRFP. Root epidermal cells were incubated in 40% sucrose to induce plasmolysis and then imaged by confocal microscopy. Bars = 1 mm in (b, g), 100 µm in (a, c–e, f, h)