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. 2018 Feb 13;8:2940. doi: 10.1038/s41598-018-21372-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histomorphological and functional analysis of H3S28S and H3S28A heart tubes. Quantification of the (a) luminal area and (b) heart muscle area over the entire cross section normalized to the luminal area (n_{H3S28S, H3S28A} = 11, 7 days old). (c) Representative cross sections of H3S28S and H3S28A heart tubes at the level of the conical chamber. Arrows mark the heart wall; asterisks mark the heart lumen. (d) Heart rate and (e) fractional shortening (FS) as a measure for the cardiac contractile function. All measurements were performed at room temperature (RT) and following thermal stimulation (37 °C) for at least five minutes (n_H3S28S = 22, n_H3S28A = 24; 2 days old). (f) Exemplary M-mode OCT registrations from H3S28S and H3S28A flies following thermal stimulation. EDD: end-diastolic diameter; ESD: end-systolic diameter.