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. 2018 Feb 13;8:2951. doi: 10.1038/s41598-018-20927-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of neurites at different positions through repaired nerves. (a) Micrographs are transverse sections showing neurofilament positive neurites at various positions in the repaired nerves (scale bar 100 μm). Neurite growth was assessed by counting the number of neurofilament-positive axons at four different positions through the repairs, proximal stump (PS), proximal device (PD), distal device (DD) and distal stump (DS). Data are means ± SEM, n = 6, showing the number of axons in each repair at the different positions (b). Two-way ANOVA with Tukey’s multiple comparisons test revealed a significant difference (P < 0.0001) in neurites between the four different positions analysed, but no significant difference between the three groups in any position.