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. 2018 Feb 13;8:2951. doi: 10.1038/s41598-018-20927-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of macrophage presence and phenotype in proximal and distal parts of the repair site. Fluorescence micrographs (a) show the presence of CD68⁺ (red) macrophages and the arginase-1 labelled sub-population of M2 phenotype cells. Hoechst staining was included to detect cell nuclei (blue). Numbers of macrophages positive for CD68 (b) and arginase-1 (c) were quantified in each transverse section, and the proportion of CD68 labelled macrophages that also expressed arginase-1 in each region was calculated (d). Data are means ± SEM, n = 6. ANOVA with Tukey’s multiple comparisons test, *p < 0.05, **p < 0.05.