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. 2018 Feb 13;8:2919. doi: 10.1038/s41598-018-21153-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mixed human-mouse two cell type experiment. (a) The number of unique aptamer reads per cell is drawn on the left. On the opposite side, all unique mRNA reads are displayed. The cells are ordered from bottom to top according to the number of assigned human mRNA reads divided by the total number of assigned mRNAs in decreasing order. (b) Two-dimensional histogram of counts per aptamer and cell. The order of the cells is the same as in (a). (c) Two-dimensional histogram of the transcript counts for the 200 most variable transcript equivalence classes. The maximum read number is truncated at 100. The order of the cells is the same as in (a,b). (d) Barnyard plot of the two cell transcriptome data. The first two principal components of the cell versus aptamer read matrix are superimposed in color space. Color code is given in the insert. The relative contribution of each aptamer to the first two principal components is given.