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. 2018 Feb 13;8:2946. doi: 10.1038/s41598-018-20920-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Minimum Evolution phylogenetic tree of 180 ST of the GT29 CAZy family. (A) The evolutionary history of the 180 ST of the GT29 CAZy family was inferred using the Minimum Evolution (ME) method. The optimal tree with the sum of branch length = 58.17312355 is shown. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the JTT matrix-based method and are in the units of the number of amino acid substitutions per site. The ME tree was searched using the Close-Neighbor-Interchange (CNI) algorithm at a search level of 1. The Neighbor-joining algorithm⁹⁷ was used to generate the initial tree. The analysis involved 180 ST amino acid sequences and all positions with less than 95% site coverage were eliminated. There were a total of 92 aa positions in the final dataset. Evolutionary analyses were conducted in MEGA7⁶⁴. Bootstrap values were calculated from 350 replicates and values greater than 50 are reported. The tree is midpoint rooted (B) Schematic ST tree summarizing the distribution of the eukaryotic ST sequences in two major groups.