Figure 3.

A54 inhibits EA‐ but not Gd³⁺‐evoked TRPC5 channel activity. Intracellular Ca²⁺ responses in HEK‐TRPC5 cells to EA (100 nM) or Gd³⁺ (30 μM) in the absence and presence of 1 μM A54 (A). (B) For EA experiments of the type illustrated in (A), mean ± SEM data normalized to the vehicle (Veh.) control for A54 (n/N = 8/32). (C) For Gd³⁺ experiments of the type illustrated in (A), mean ± SEM data normalized to the Veh. control for A54 (n/N = 8/32). The time point at which the amplitude of the Gd³⁺ response was measured was from 150 to 200 s. (D) Activation time course of Gd³⁺ responses in the absence and presence of A54. The curves were fitted exponential functions with time constants of 780 ± 7.6 s (Gd³⁺ − A54) and 18.1 ± 3.9 s (Gd³⁺ + A54) (n/N = 8/32).