Table 1.
Substrate for TycA-A | Nucleophile | aProduct (mM) | bConversion (%) | Protonated parent ion (m/z) | MS/MS fragment ion (m/z) | |||
---|---|---|---|---|---|---|---|---|
cType 1 | dType 2 | |||||||
l-Tryptophan | (Linear amines) | |||||||
Methylamine | 0.81 | 8.1 | 218.1302 | 58.0300 | 159.0923 | 87.0552 | 130.0655 | |
Dimethylamine | 0.81 | 8.1 | 232.0858 | 72.0446 | 159.0915 | 101.0705 | 130.0648 | |
β-Alanine | 0.96 | 9.6 | 276.1371 | 116.0508 | 159.0930 | 145.0653 | 130.0674 | |
γ-Aminobutyric acid | 1.16 | 11.6 | 290.1532 | 130.0663 | 159.0934 | ND | ND | |
(Cyclic amines) | ||||||||
Azetidine | 0.82 | 8.2 | 244.1457 | 84.0450 | 159.0927 | 113.0713 | 130.0657 | |
Pyrrolidine | 0.92 | 9.2 | 258.1301 | 98.0598 | 159.0916 | 127.0863 | 130.0646 | |
Piperidine | 1.40 | 10.4 | 272.1783 | 112.0767 | 159.0930 | 141.1038 | 130.0661 | |
Azepane | 0.52 | 5.2 | 286.1944 | 126.0927 | 159.0933 | 155.1198 | 130.0663 | |
Azocane | 1.35 | 13.5 | 300.2090 | 140.1087 | 159.0936 | 169.1350 | 130.0665 | |
(Cyclic amino acids and derivative) | ||||||||
d-Proline | 0.46 | 4.6 | 302.1566 | 142.0647 | 159.0917 | 171.0760 | 130.0647 | |
cis-4-Hydroxy-l-proline | 0.94 | 9.4 | 318.1136 | ND | 159.0910 | 187.0707 | 130.0642 | |
cis-4-Hydroxy-d-proline | 0.78 | 7.8 | 318.1481 | ND | 159.0934 | 187.0742 | 130.0662 | |
l-Prolinamide | 0.85 | 8.5 | 301.1682 | ND | 159.0940 | 170.0626 | 130.0666 | |
l-Azetidine-2-carboxylic acid | 0.78 | 7.8 | 288.1772 | 128.0342 | 159.0918 | 157.0604 | 130.0649 |
ND, not detected
aCalculated from the amount of decrease in l-tryptophan minus the amount of l-tryptophyl-l-tryptophan generation.
bCalculated against the added l-tryptophan concentration.
cFragment ion generated by C1-C2 cleavage.
dFragment ion generated by C2-C3 cleavage.