TABLE 4 .
Designation | Gene | rpoZ/WT | Ratioa | Function | Prophage |
---|---|---|---|---|---|
1 | yagM | 10.29 ± 0.5 | 0.0972 | Phage protein with unknown function | CP4-6 |
2 | yffL | 4.48 ± 0.5 | 0.2233 | Phage protein with unknown function | CPZ-55 |
3 | yagN | 2.44 ± 0.6 | 0.4093 | Phage protein with unknown function | CP4-6 |
4 | ymfJ | 1.62 ± 0.6 | 0.6184 | Phage protein with unknown function | e14 |
5 | yagL | 1.54 ± 0.6 | 0.6405 | Predicted transcription factor | CP4-6 |
6 | intF | 1.47 ± 0.5 | 0.6816 | Putative phage integrase | CP4-6 |
7 | nmpC | 0.99 ± 0.9 | 1.0141 | Putative OM protein | DPL |
8 | afuB | 0.86 ± 0.6 | 1.1638 | Predicted ferric transporter | CP4-6 |
a | yjhH | 0.95 ± 0.7 | 1.0494 | Putative lyase | KpLE2 |
b | ykfC | 0.87 ± 0.5 | 1.1436 | Phage protein with unknown function | CP4-6 |
Ratio indicates the relative level of mRNA (rpoZ mutant/wild type). When the target gene is located inside a prophage, the name of the prophage is given.
The level of mRNA was determined for E. coli K-12 mutants lacking the rpoZ gene by using the qRT-PCR method. The genes were selected from the list showing the decreased distribution of RpoZ-defective RNAP (Table 3 and Fig. 3). As references, two genes (a and b) were selected from the list of genes with increased distribution of RpoZ-defective RNAP (Table 2 and Fig. 2).