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. 2018 Feb 13;3(1):e00172-17. doi: 10.1128/mSystems.00172-17

TABLE 4 .

Transcription of genes that showed decreased distribution of RpoZ-defective RNAP inside prophages (qRT-PCR assay)b

Designation Gene rpoZ/WT Ratioa Function Prophage
1 yagM 10.29 ± 0.5 0.0972 Phage protein with unknown function CP4-6
2 yffL 4.48 ± 0.5 0.2233 Phage protein with unknown function CPZ-55
3 yagN 2.44 ± 0.6 0.4093 Phage protein with unknown function CP4-6
4 ymfJ 1.62 ± 0.6 0.6184 Phage protein with unknown function e14
5 yagL 1.54 ± 0.6 0.6405 Predicted transcription factor CP4-6
6 intF 1.47 ± 0.5 0.6816 Putative phage integrase CP4-6
7 nmpC 0.99 ± 0.9 1.0141 Putative OM protein DPL
8 afuB 0.86 ± 0.6 1.1638 Predicted ferric transporter CP4-6
a yjhH 0.95 ± 0.7 1.0494 Putative lyase KpLE2
b ykfC 0.87 ± 0.5 1.1436 Phage protein with unknown function CP4-6
a

Ratio indicates the relative level of mRNA (rpoZ mutant/wild type). When the target gene is located inside a prophage, the name of the prophage is given.

b

The level of mRNA was determined for E. coli K-12 mutants lacking the rpoZ gene by using the qRT-PCR method. The genes were selected from the list showing the decreased distribution of RpoZ-defective RNAP (Table 3 and Fig. 3). As references, two genes (a and b) were selected from the list of genes with increased distribution of RpoZ-defective RNAP (Table 2 and Fig. 2).