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. 2018 Feb 9;9:130. doi: 10.3389/fpls.2018.00130

FIGURE 3.

FIGURE 3

Protein expression analysis (Western blot) with anti-GFP antibodies, anti-rabbit peroxidase, and ECL (A) and anti-cmyc antibodies, anti-mouse peroxidase, and ECL (B), of transgenic lines expressing NSlmb-VHHGFP4 and GFP (5II/40, 5II/45, 8/20, 8/24), NSnoFbox-VHHGFP4 and GFP (L7/2, L7/6), and GFP (GFP) and of tobacco wild-type (WT) plants. NSnoFbox-VHHGFP4 (fusion protein with deleted F-box domain) and WT plants served as controls. The size of molecular marker proteins is given in kilodalton. The expected molecular weights of GFP, NSlmb-VHHGFP4, and NSnoFbox-VHHGFP4 are 27, 39, and 35 kDa, respectively. Extracts of 5II/40, 5II/45, 8/20, 8/24, and GFP have been treated with MG-132 (+) or not (–). In both cases GFP protein expression is lacking in 5II/40, 5II/45, 8/20, and 8/24. eGFP, pure eGFP.