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. 2016 May 9;15(2):120–134. doi: 10.1038/cmi.2016.21

Figure 4.

Figure 4

GRA15II-polarized RAW264.7 cells exhibited decreased expression of the profibrogenic factor profile in vitro. GRA15II-RAW264.7 weakened JS1 fibrogenic activities and ROP16I/III-RAW264.7 control cells promoted the activities based on the measurement of mRNA (a–c) and protein (d) relative expression levels of α-SMA, Col I and TGF-β1 in the JS1 cells after co-culturing. No significant difference was detected between JS1 cells co-cultured with LV-RAW264.7 and RAW264.7 cells. JS1 cells produced a decreased level of α-SMA and Col I when co-cultured with GRA15II-RAW264.7 cells and an enhanced level when co-cultured with ROP16I/III-RAW264.7 cells compared with the LV-RAW264.7 control cells. Simultaneously, escalated TGF-β1 expression was noted in the ROP16I/III-RAW264.7 group (*P<0.05, ***P<0.001, n=4). Col I, type 1 collagen; α-SMA, α-smooth muscle actin; TGF-β1, transforming growth factor beta 1.