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. 2017 Jan 16;15(2):171–181. doi: 10.1038/cmi.2016.60

Figure 1.

Figure 1

(a) RA-FLSs expressed higher levels of uPAR than OA and trauma groups. Western blot assays were used to detect uPAR expression in RA, OA, and traumatic FLSs. Densitometry analysis of protein expression (means±s.e.m.) were representative of three independent experiments. (b) uPAR-siRNA efficiently interferes with uPAR expression in RA-FLSs. Cells were transfected with uPAR-siRNA (40 nM) or a negative control (NC, 40 nM) for 48, 72, 96 h and western blot analysis was used to detect the effect of uPAR-siRNA on uPAR expression. Upper panel, representative images are shown; lower panel, quantification (means±s.e.m.) of three independent experiments normalized to 1 in the untreated sample. (c) uPAR knockdown affects suPAR/uPA secretion. Secretion curves of suPAR/uPA in the supernatants of transfected RA-FLSs for 48 , 72 , 96 h was made by ELISA assays of three independent experiments. *P<0.05, compared with the controls and n=4. FLSs, fibroblast-like synoviocytes; OA, osteoarthritis; uPAR, Urokinase-type plasminogen activator receptor.