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. 2017 Sep 11;15(2):99–110. doi: 10.1038/cmi.2017.82

Figure 3.

Figure 3

LPS stimulation downregulates miR-151-3p expression and upregulates Stat3 protein levels in macrophages. (a) RAW264.7 cells and primary peritoneal macrophages were stimulated with or without 100 ng/ml LPS for the indicated time points. The expression of miR-151-3p was measured using RT-qPCR and normalized to the expression of U6. (b) Bone marrow-derived macrophages (BMDM), bone marrow-derived dendritic cells (BMDC), mouse embryo fibroblasts (MEF) and the colon cancer cell line CT-26 were stimulated with or without 100 ng/ml LPS for the indicated time points. The expression of miR-151-3p was measured using RT-qPCR and normalized to the expression of U6. (c and d) The levels of p-Stat3 and Stat3 protein (c) and Stat3 mRNA (d) were analyzed by western blotting and RT-qPCR in macrophages stimulated with or without LPS (100 ng/ml) as indicated. Data are shown as the means±s.d. (n=3) of one representative experiment. Similar results were obtained in at least three independent experiments. *P<0.05; **P<0.01, unpaired Student's t-test.