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. 2017 Sep 11;15(2):99–110. doi: 10.1038/cmi.2017.82

Figure 5.

Figure 5

LPS activates Stat3 in an IL-6 independent manner. (a) Primary macrophages from IL-6−/− and WT mice were treated with LPS (100 ng/ml) for the indicated time points. p-Stat3 and Stat3 were analyzed using immunoblotting. (b) Primary macrophages were treated with IL-6 antibody or mouse normal IgG and subsequently treated with LPS for the indicated time points. p-Stat3 and Stat3 were analyzed through immunoblotting. (c–e) Primary macrophages were treated with CHX, ACTD or BFA for eight hours and then stimulated with LPS (100 ng/ml) for the indicated time points. p-Stat3 and Stat3 levels were analyzed using immunoblotting.