Table 1.

Sequences of primers used in quantitative real time-polymerase chain reaction (qRT-PCR) experiments, the annealing temperatures for PCR amplification of each gene that were investigated, and the amplicon lengths

SYBR Green RT-PCR Primers	Sense primer(5’ toward 3’)	Anti-sense primer(5’ toward 3’)	Annealing Temperature (°C)	Amplicon length (bp)
β-actin	CACACCCGCCACCAGTTC	GACCCATACCCACCATCACAC	60	166
Tnf-α	AAATGGGCTCCCTCTCATCAGTTC	TCCGCTTGGTGGTTTGCTACGAC	60	180
Il-6	GACTTCCAGCCAGTTGCCTTCTTG	TGGTCTGTTGTGGGTGGTATCCTC	60	111

The primers are shown for the sequences of rat Il-6: interleukin-6; TNF-α: tumor necrosis factor-α, β-actin, and beta-actin. β-actin was selected as the reference gene