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. 2018 Aug 22;3:5. Originally published 2018 Jan 12. [Version 2] doi: 10.12688/wellcomeopenres.13387.2

Figure 6. Filtered HSQC spectroscopy.

Figure 6.

The application of a 15N filtering block in the 1H, 13C-HSQC pulse sequence ( A) allows the observation of 1H, 13C groups directly coupled to 15N nuclei. In the sequence in panel B-1 no filtering will be observed and the resulting spectrum ( C-1) will contain all 1H, 13C groups adjacent to either 14N or 15N nuclei. The use of a 15N filter ( B-2) will result in only those 1H, 13C groups adjacent to a 15N nuclei being observed in the resulting 1H, 13C HSQC spectrum ( C2). 1H, 13C J-coupling is suppressed during acquisition using adiabatic bilevel decoupling (ad-bilev) 21. The pulse phases are: ϕ 1 = y; ϕ 2 = x, -x; ϕ 3 = x for the no filter sequence (panel B-1) and y for the 15N filtered sequence (panel B-2); ϕ 4 = y, –y for the no filter sequence and y, y for the 15N filtered sequence; ϕ 5 = y, -y for the no filter sequence x, -x for the 15N filtered sequence; ϕ 6 = x, x, -x, -x; ϕ 7 = x, x, x, x, -x, -x, -x, -x; ϕ 8 = x, x, x, x, y, y, y, y; ϕ rec = x, -x, -x, x, y, -y, -y, y.