Table 1. Comparison of spectroscopic techniques.

The acquisition time and signal to noise ratios of various experiments used in this study. A good signal to noise ratio can be achieved using the spectral filtering allowing rapid measurement of quantitative spectra. The signal to noise benefit of the HSQC over the 1D ¹³C acquisition is clearly seen. The effect of increasing the J-coupling splitting enhancement whilst simultaneously reducing the number of increments on the acquisition time of ¹H, ¹³C-HSQC spectra is also shown.

Experiment	Acquisition time [mins]	Signal to noise ratio (CH 3 of lactate)	Transients	TD	SW [ppm]	Splitting enhancement
1H 1D (all 1H)	2	327.31	8	16384	12	1
1H 1D ( 13C bound 1H)	2	66.13	8	16384	12	1
1H, 13C-HSQC	233	823.86	2	1024/8192	12/160	1
1H, 13C-HSQC	110	770.98	2	1024/4096	12/160	2
1H, 13C-HSQC	51	426.41	2	1024/2048	12/160	4
1H, 13C-HSQC	24	283.38	2	1024/1024	12/160	8
13C 1D (30° excitation)	1414	59.95	16384	65538	239	1