Figure 1.

Identification of human ILC2s by flow cytometry as conducted in our studies. PBMCs were isolated from human peripheral blood and analysed by multifluorochrome‐based flow cytometry. PBMCs were gated on leucocytes (A), single cells (B) and live cells using a viability dye (C). Live single cells were gated on lineage negative (CD3, CD14, CD16, CD19, CD20, CD56, CD123, CD11c, αβTCR γδTCR), CD45+ (D), CD127+ (E) and CD161+CRTH2+ cells (F), which finally leads to the identification of lin‐CD45+CD127+CRTH2+CD161+ ILC2s