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. 2018 Feb 9;9:109. doi: 10.3389/fpls.2018.00109

FIGURE 1.

FIGURE 1

Generation of Setaria AGG3 overexpression lines. (A) Map of the expression vector used for cloning a monocot codon optimized Arabidopsis AGG3 gene. (B) Identification of the homozygous families from single insert AGG3-4A transgenic plants in T1 generation using Taqman assay (C) Expression analysis of AGG3 transgene and endogenous Setaria G-protein genes by qRT-PCR analysis. Transcript levels of indicated genes were determined in EV and AGG3-OE (A1 and A4) lines using cDNA isolated from 10-day-old S. viridis seedlings. The expression values of EV, A1 and A4 were normalized with Setaria ubiquitin gene. Dotted line represents the expression level of SvGγ3a, SvGγ3b, SvGγ3c, SviGα1, SvGβ1, SvGγ1, SvGγ1a, SvGγ2, SvRGS in the EV (assigned as 1). The values are presented as the mean ± SE of three biological replicates and represented as fold changes. EV, Setaria plants overexpressing the empty vector; A1, A4 are the two independent transgenic lines for AGG3 overexpression (AGG3-OE).