Skip to main content
. 2018 Feb 9;9:109. doi: 10.3389/fpls.2018.00109

FIGURE 2.

FIGURE 2

Effect of different abiotic stresses on early development of Setaria AGG3OE plants. (A) Percentage of seed germination in EV, A1, and A4 transgenic seeds under control conditions. (B) Percentage of seed germination in EV, A1 and A4 transgenic seeds in the presence of 0.5 X MS media containing 0.5 μM ABA or (C) 3% glucose. For these experiments, 24 seeds per genotype, per treatment were used. Seeds were sterilized and stratified at 4°C for 2 days. Seed germination was calculated as the percentage of total seeds that germinated after 5 days post-stratification. The data represent mean values (±SE) of three biological replicates. (D) Post-germination seedling growth of EV and A1 and A4 transgenic plants in the presence of 2 μM ABA, (E) 3% glucose or (F) 100 mM NaCl. Seeds were germinated on 0.5 X MS agar for 2 days followed by their transfer to the media containing different additives and grown for another 3 days. Root and coleoptile lengths were measured. The graphs represent the mean values (±SE) from sixty seedlings. The corresponding representative pictures are also shown. Asterisks represent P-values ≤ 0.05 (∗), ≤0.01 (∗∗), or ≤0.001 (∗∗∗) as calculated using Students t-test.