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. 2018 Feb 9;9:109. doi: 10.3389/fpls.2018.00109

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Copyright © 2018 Kaur, Roy Choudhury, Vijayakumar, Hovis, Rhodes, Polzin, Blumenthal and Pandey.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of nitrogen limiting conditions on growth of AGG3OE Setaria plants. (A) Root and coleoptile lengths of 1 week old seedling grown on 0.5 X MS media lacking nitrogen or phosphate sources. The data represent the mean values (±SE) from sixty seedlings. Asterisks represent P-values ≤ 0.01 (^∗∗) or ≤0.001 (^∗∗∗) as calculated using Students t-test. (B) Representative picture of 2 weeks old seedlings of EV control and AGG3OE lines grown on nitrogen free media. (C) Representative picture of the flag leaf of 5 weeks old EV, A1 and A4 plants under nitrogen-limiting condition. (D) Expression levels of nitrate transporter and metabolism genes in 2-week old S. viridis A1 and A4 plants. Dotted line represents the expression values of the genes (assigned as 1) in EV control plants. The data represent mean values (±SE) of three biological replicates.