Figure 1. Overview of kill switch design concept.
A) In permissive conditions the toxin is repressed. The antitoxin is expressed at a constitutive low level to accommodate for any leaky expression of the toxin. B) Upon a change of environment to non-permissive conditions the repression is lifted and toxin expression increases. The low level of antitoxin expression is no longer capable of preventing a lethal level of free toxic