Skip to main content
. Author manuscript; available in PMC: 2018 Feb 14.
Published in final edited form as: Mol Cell. 2017 Nov 16;68(4):686–697.e3. doi: 10.1016/j.molcel.2017.10.033

Figure 5. Design of the temperature sensitive kill switch cryodeath.

Figure 5

A) At 37 °C translation of CcdB is limited allowing cell survival. B) At colder temperatures, expression of CcdB increases, resulting in cell death C) The engineered region encompassing the regulatory DNA for CcdB and CcdA. Highlighted are segments that have a strong impact on expression level for both the toxin (red) and antitoxin (green). Varied bases are emphasized. Y = C or T; M = A or C; W = A or T; R = A or G. See also Figure S3.