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. 2018 Feb 13;49:15. doi: 10.1186/s13567-018-0508-z

Figure 2.

Figure 2

Selenium blocks the PCV2 replication promoted by OTA. PK-15 cells were cultured for 12 h with selenium at various concentrations and then treated with PCV2 in the presence or absence of 0.1 μM OTA for another 60 h. Cells were assayed for A PCV2 viral cap protein expression by Western blotting, B PCV2 viral DNA copies by Quantitative real-time PCR, C virus titers and D the number of infected cells (Scar bar: 100 μm) by IFA as described in Materials and methods. Data are presented as mean ± SE of three independent experiments. Significance compared with control, *P < 0.05 and **P < 0.01. Within the OTA-treated groups, significance compared with cells without selenium, #P < 0.05 and ##P < 0.01.